The innovative Leica EM Cryo CLEM set for widefield lightmicroscopy with the Leica DM6 FS represents the state-of-the art solution for analysis of cryo samples in the cryo light and electron microscope.
For easy, contamination-free sample transfer from cryo sample preparation instruments, the cryo transfer shuttle is docked to the Leica EM Cryo stage mounted to the microscope. The Leica EM Cryo CLEM objective with low working distance (< 0,28 mm) for high resolution ensures fast and specific localization of target structures in the light microscopy leading to fast and accurately determine regions of interest in the electron microscope.
Microscope stand
- DM6 FS
Camera
- Leica DFC9000 GT: deep-cooled 4.2 MP sCMOS camera with a maximum of 82% quantum efficiency, 50 frames per second acquisition speed, and extreme low dark current of 0.14e/p/sec, pixel size: 6,5 μm, Bit depth: 12/16 bit.
Objective lens
- Leica HCX PL APO 50x / 0.90 CLEM objective. Working distance of 0.28 mm. Maximum resolution of 512 nm.
Filter system
- DAPI ET: Excitation: BP 350/50; Dichroic: LP 400, Emission: BP 460/50
- GFP ET: Excitation: 470/40; Dichroic: 495; Emission: 525/50
- TXR ET: Excitation: BP 560/40; Dichroic: LP 585; Emission: BP 630/75
Software
- LAS X Cryo CLEM
- CLEM Viewer
- Thunder module
